【Keyword】 Japanese sake ethanol recovery rate improvement fractionation Conveni-Prep
・Green tea in a plastic bottle
<Basic operation>
<Evaluations>
① Linearity of the adsorpted amount of polyphenol fraction
In order to evaluate the adsorbance ability of the adsorbent, the amount of the polyphenol fraction
when the sample amounts are 10 to 40 mL were confirmed .
②Repetitive use evaluation of adsorbent
The polyphenol fraction amount was confirmed when the adsorbent was repeatedly used.
① Linearity of the adsorped amount of polyphenol fraction
The weight of the polyphenol fraction after evaporation to dryness was measured when the sample volume was 10 to 40 mL.
As shown in Tab. 1 and Fig. 4, there was a fine correlation between the sample amount and the obtained polyphenol fraction amount. It was confirmed that the adsorb ability of this adsorbent is good enough to be used for the quantitative solid phase extraction work. Also, when 20 mL of tea with a high catechin content was treated, more than 100 mg the polyphenol fraction can be obtained.
②Repetitive use evaluation of adsorbent
Tab. 2 and Fig. 5 show the result when the sample amount was set as 20 mL, and the same adsorbent was repeatedly used. The result of six times repeated use showed relatively fine value, average=19.8 mg and C.V.=3.9%, that meant the adsorbent used in this experiment is sufficiently tolerable with repetitive use.
Fig. 6 shows the HPLC charts of the analysis of polyphenol fractions obtained in first and sixth experiments. It was confirmed that almost same components were obtained as the phenol molecules obtained by the repeated experiments.
HPLC conditions
Column:Inertsil ODS-4 (3.0×100mm)
Mobile phase:A: 50mmol/L NaH₂PO₄ (pH₄)
B:CH₃CN
Gradient :Time(min) 0 ⇒ 15
B(%) 10 ⇒ 45
Flow rate :0.3mL/min
Column temperature:40°C
Detection wavelength:270nm
By using 60 mL reservoir with "Conveni-Prep" , medium volume samples can be processed at once. Since the discharge from the reservoir is carried out mechanically, easy and quick fractionation of polyphenols from green tea is possible.
Also as the evaporation solidification work following to the extraction can be done quickly and easily, this instrument is hoped to be applicated for such works as fractionations of the other active ingredients.
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