Customer interview at Prince of Songkla University as to the benefits of the Smart Evaporator C10
Location of the unit :
Department of Pharmacognosy and Pharmaceutical Botany,
Prince of Songkla University (Thailand)
Interviewed with :
Ms. Similan Inaugsorn
-Master degree student , Department of Pharmacognosy and Pharmaceutical Botany.
Name/Quantity of Solvent : 85% Ethanol
Type of container : 2 ML vial HPLC
Temperature setting : 70°C
Operation hour : 10-15 min
Mr. Adisak Muakdang
-Research Assistant , Department of Pharmacognosy and Pharmaceutical Botany.
Name/Quantity of Solvent : DMSO
Type of container : 2 ML vial HPLC
Temperature setting : without heating
Operation hour : 9 hours
Q：What is your research ?
A：Ms. Similan : Analysis of andrographolide in Andrographis extraction ( or in thailand called Fa thalai chon)
Mr. Adisak : Analysis of Mitragynine and 7-hydroxy-mitragynine in Kratom leaf extract. Mostly, we research about Local herb extraction. We analyze the active ingredient in the Local herb.
Q：Could you please tell us a little bit about your process?
A：Ms. Silmilan : I extract the Andrographis by using reflux extraction with 85% ethanol.
After Reflux Extraction, I use Smart Evaporator C10 to evaporate out the solvent. Then I use HPLC to analyze the quantitative of andrographolide with water/acetonitrile as a mobile phase.
Q：That’s reason why you have to evaporate out the ethanol.
A：Ms. Silmilan : Yes, we use a different solvent so we have to evaporate all of the ethanol before HPLC.
Q：How about you Mr. Adisak ?
A：Mr. Adisak : In order to extract 7-hydroxy-mitragynine, we can not be use the chloroform
because 7-hydroxy-mitragynine can be decomposed in chloroform. We have to use DMSO as a solvent instead. In order to evaporate out the DMSO, we have to control the temperature to not over 50°C.
Q：What is your method before using Smart Evaporator C10 ?
A：Mr. Adisak : Before using the Smart Evaporator C10, I used to work with Freeze dry machines before.
Ms. Similan : Previously using Rotary Evaporator, it took more time. (Takes about 30-40 minutes) Compared to using the Smart Evaporator C10 it takes only 10-15 minutes and can do 10 samples at once.
Q：What is your benefit you get from using Smart Evaporator C10 ?
A：Ms. Silmilan : We finish the job faster! We are concerned about the timing for our sample because the active
ingredient will decompose, if the laboratory took a long time. We have to complete the laboratory within one day. If we use Rotary Evaporator, we can do one by one sample and it would take a long time. When we use Smart Evaporator C10, we can do 10 samples in time so it can be completed in a day.
Mr. Adisak : Compared to the use of Freeze dry, it can save the cost of using nitrogen gas. It took me 9 hours, but I was able to do other things in the meantime.
■Interested in what Smart Evaporator is? You can learn from here!
■Product details of Smart Evaporator C10 (10 channel evaporator)
All Biochromato products must be for Research Use Only. It is not to be used for clinical or diagnostic use, nor food processing or storage. To resell the products, a separate agreement is required.