【Keyword】 Japanese sake ethanol recovery rate improvement fractionation Conveni-Prep
・Japanese Sake (Dai-ginjo quality, Alcohol content:15~16%)
<Basic operation>
<Evaluations>
① Influence of "suction agitating time" in recovery rate
Confirmed the amount of solvent extraction fraction when the suction agitating time is varied from 0 to 90 min.
② Evaluation of solvent extraction fractionation components
The solvent extraction fractions obtained at two different suction agitating times "none" or "90 minutes“ are analyzed
by thermal desorption / pyrolysis DART®-MS and are compared.
① Influence of "suction agitating time" in recovery rate
Tab.1 and Fig.4 show the amount of the dried and solidated fractions finally obtained by the solvent extraction. The time of "suction
agitating", which is carried out when the sample is added to the solid phase (adsorbent), is varied. As a result, the amount of fractions
increased by "suction agitating“; the yield increased about twice at 90 min. With the use of “Conveni-Prep” , "suction agitating “ can be
carried out while the solid phase extraction process. It easily makes the improvement of recovery rate for the solvent extracted fractions
from the alcohol containing samples.
② Evaluation of solvent extraction fractionation components
The obtained solvent extracted fraction was analyzed by thermal desorption / pyrolysis DART®-MS . There is no apparent deference
between the components obtained with or without “suction agitating”(MS spectrum in the Fig.6 upper) . However, when compared by
the chromatogram (EIC) of individual peaks, there is a component whose extraction amount does not change (eg: m/z 226) or
components whose extraction amount increase (for example, m/z 272, 434).
(Supplement)Thermal desorption / pyrolysis DART®-MS
The sample is vaporized by heating the sample linearly from room temperature to 600°C. Since the components in the sample are directly ionized for mass spectrometry, simultaneous analysis can be performed without treatment even for samples having complicated compositions. Fig.7shows the system outline. It is also a feature of the system that it has quantitative ability.
In addition to alcoholic beverages, this method will be useful for increasing of the fractionation yield for alcohol extracts from food samples, culture media, etc. (Increase in yield of precious components, simplifying pretreatment ).
All Biochromato products must be for Research Use Only. It is not to be used for clinical or diagnostic use, nor food processing or storage. To resell the products, a separate agreement is required.