Improvement of fraction recovery rate from alcohol-containing sample

~Japanese Sake~

【Keyword】 Japanese sake　ethanol　recovery rate improvement　fractionation　Conveni-Prep　

Introduction

• • As it is said that “sake is the chief of medicine” since ancient times, Japanese sake contains many active ingredients.
  • Solid phase extraction is often used for discovery work for active ingredients in Japanese sake. However, when Japanese sake is used as it is as a sample without pretreatment, there is a problem that the yield at fractionation decreases by the influence of alcohol.
  • It is possible to improve the yield of fractionation from the sample containing alcohol by using ” Conveni-Prep” , which is a fractionation system utilizes the patented VVC method* for suction agitating at the sample application.
    ＊Vacuum Vortex Concentration : VVC, Fig.2

Mechanism of suction vaporization solid phase extraction method

• • This device is a fractionation system using the patented VVC method, and it is possible to concentrate and dry the fractionated sample as it is in one device.
  • The flow of each step of the fractionation work is shown in Fig.1. When the sample is placed in
    the reservoir filled with the adsorbent, "suction agitating" is performed by the VVC method to
    promote vaporization of the alcohol content in the sample, resulting in reduction of alcohol concentration (Fig. 1 red line part).
  • Since the adsorption amount which has been influenced by the alcohol content can be increased , the fractionation recovery rate can be improved.

Sample

・Japanese Sake （Dai-ginjo quality, Alcohol content：15～16%）

Experiment

<Basic operation>

• Add 10 g of aromatic synthetic adsorbent to the reservoir and perform conditioning with methanol.
• After adding 20 mL of sample to the reservoir, perform "suction agitation" for a certain period of time (suction amount: 25 L / min).
• Discharge the sample from reservoir.
• Add 20 mL of water to the reservoir and wash the adsorbent.
• Add 15 mL of methanol to the reservoir and receive the solvent extraction fraction in a vial.
• Concentrate and dry solidification by vortex and suction while maintaining the extract solution in the vial at 50 ° C.